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phosphorylated oligo duplex  (New England Biolabs)


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    New England Biolabs phosphorylated oligo duplex
    Phosphorylated Oligo Duplex, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+oligo/BbsI/pmc12652319-269-6-19
    Average 98 stars, based on 1922 article reviews
    phosphorylated oligo duplex - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters
    Article Snippet: .. In a 1.8 mL microcentrifuge tube, prepare an annealing reaction between the custom 5’-phosporylated oligo and the ssDNA, using a 4:1 molar ratio of phosphorylated oligo to single stranded DNA with 1X NEBuffer 2.1 as shown in the example below: Example: To anneal phosphorylated oligo to 100 μg (~91 pmol) ssDNA Add 50 μL single stranded DNA (initial concentration 2000 ng/μL; final concentration 833 ng/μL) Add 12 μL 10X NEBuffer 2.1 (1X) Add 3.6 μL 100μM custom oligonucleotide (360 pmol; 16 μM) Add 54 μL DEPC-treated water (up to a total volume of 120 μL) Mix thoroughly by pipetting. ..



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